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Secondary Antibodies

Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies (44,201)
Mouse Secondary Antibodies (14,525)
Rabbit Secondary Antibodies (6,310)
Donkey Secondary Antibodies (4,319)
Rat Secondary Antibodies (1,237)
Sheep Secondary Antibodies (701)
Chicken Secondary Antibodies (403)
Bovine Secondary Antibodies (257)
Pig Secondary Antibodies (77)
Horse Secondary Antibodies (74)
Alpaca Secondary Antibodies (62)
Hamster Secondary Antibodies (57)
Biosimilar Secondary Antibodies (9)
Human Secondary Antibodies (7)
Duck Secondary Antibodies (3)

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4,756 – 4,770 of 72,047 results
4,756

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 594, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Rabbit
Host Species Donkey
Conjugate DyLight 594
Applications Flow Cytometry,Immunocytochemistry/Immunofluorescence,Immunomicroscopy
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Flow Cytometry 1:20 - 1:2000, Immunocytochemistry/Immunofluorescence, Immunomicroscopy 1:20 - 1:2000
Classification Polyclonal
Immunogen Purified Rabbit IgG (H&L)
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on rabbit IgG and light chains on all rabbit immunoglobulins
4,757

Donkey anti-Goat IgG (H+L) Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Goat
Host Species Donkey
Conjugate HRP
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Goat IgG (H&L)
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on goat IgG and light chains on all goat immunoglobulins. This antibody has been pre-adsorbed against human, mouse, rabbit and rat IgG.
4,758

Donkey anti-Human IgG (H+L) Secondary Antibody, DyLight 755, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Human
Host Species Donkey
Conjugate DyLight 755
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50 mM Sodium Borate
Classification Polyclonal
Immunogen This Donkey anti-Human IgG (H+L) Secondary Antibody was developed against human IgG-heavy and light chain.
Primary or Secondary Secondary
4,759

Donkey anti-Goat IgG (H+L) Secondary Antibody, Novus Biologicals™
SDP

Donkey Polyclonal Antibody has been used in 8 publications

Content And Storage Store at 4C. Do not freeze.
Target Species Goat
Host Species Donkey
Conjugate Unconjugated
Applications Adhesion Activation,ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Frozen),Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.0 mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:1, ELISA 1:1, Immunohistochemistry 1:200-1:2000, Immunocytochemistry/Immunofluorescence 1:200 - 1:2, Immunohistochemistry-Paraffin 1:200 - 1:2000, Immunohistochemistry-Frozen 1:200-1:2000, Adhesion Activation
Classification Polyclonal
Immunogen Goat IgG-heavy and light chain
Primary or Secondary Secondary
Test Specificity By immunoelectrophoresis and ELISA this reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
4,760

Donkey anti-Rat IgG (H+L) Secondary Antibody [Janelia Fluor 669], Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rat
Host Species Donkey
Conjugate Janelia Fluor 669
Applications Western Blot,ELISA,Immunohistochemistry
Form Purified
Isotype IgG
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen Rat IgG whole molecule
Primary or Secondary Secondary
4,761

Donkey anti-Mouse IgG (H+L) Secondary Antibody, Alkaline Phosphatase (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles.
Target Species Mouse
Host Species Donkey
Conjugate Alkaline Phosphatase (AP)
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.50mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Mouse IgG (H&L)
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on mouse IgG and light chains on all mouse immunoglobulins. This antibody has been pre-adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat and sheep IgG.
4,762

Donkey anti-Mouse IgG (H+L) Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Mouse
Host Species Donkey
Conjugate HRP
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Mouse IgG (H&L)
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on mouse IgG and light chains on all mouse immunoglobulins. This antibody has been pre-adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat and sheep IgG.
4,763

Donkey anti-Rat IgG (H+L) Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Rat
Host Species Donkey
Conjugate HRP
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Rat IgG (H&L)
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on rat IgG and light chains on all rat immunoglobulins. This antibody has been pre-adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit and sheep IgG.
4,764

Donkey anti-Sheep IgG (H+L) Secondary Antibody, Biotin, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Sheep
Host Species Donkey
Conjugate Biotin
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation PBS
Classification Polyclonal
Immunogen This Donkey anti-Sheep IgG (H+L) Secondary Antibody was developed against sheep IgG-heavy and light chain.
Primary or Secondary Secondary
4,766

Donkey anti-Sheep IgG (H+L) Secondary Antibody, DyLight 405, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Sheep
Host Species Donkey
Conjugate DyLight 405
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen This Donkey anti-Sheep IgG (H+L) Secondary Antibody was developed against sheep IgG-heavy and light chain.
Primary or Secondary Secondary
4,767

Donkey anti-Sheep IgG (H+L) Secondary Antibody, Alkaline Phosphatase, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Sheep
Host Species Donkey
Conjugate Alkaline Phosphatase (AP)
Applications ELISA,Immunohistochemistry,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Dilution Western Blot 1:1000 - 1:4000, ELISA 1:2000 - 1:8000, Immunohistochemistry 1:200 - 1:1000
Classification Polyclonal
Immunogen Donkey anti-Sheep IgG (H+L) Secondary Antibody [Alkaline Phosphatase] was produced by repeated immunization with Sheep IgG whole molecule in donkey.
Primary or Secondary Secondary
Test Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Sheep IgG and Sheep Serum.
4,768

Donkey anti-Sheep IgG (H+L) Secondary Antibody, Rhodamine, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Target Species Sheep
Host Species Donkey
Conjugate Rhodamine
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Dilution Flow Cytometry 1:500 - 1:2500, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Classification Polyclonal
Immunogen Sheep IgG whole molecule
Primary or Secondary Secondary
Test Specificity Sheep IgG (H and L)
4,770

Donkey anti-Mouse IgG (H+L) Secondary Antibody, Biotin (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Target Species Mouse
Host Species Donkey
Conjugate Biotin
Applications ELISA,Immunohistochemistry,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.0 mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Dilution Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000
Classification Polyclonal
Immunogen Mouse IgG whole molecule
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat, and Sheep Serum Proteins. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.